pcag mito mtagbfp2 (Addgene inc)
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pcag mito mtagbfp2
Pcag Mito Mtagbfp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcag+mito+mtagbfp2/pCAG+mito-mTagBFP2+(Plasmid+%23105011)/pmc13030966-223-20-28
Average 92 stars, based on 6 article reviews
Pcag Mito Mtagbfp2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcag+mito+mtagbfp2/pCAG+mito-mTagBFP2+(Plasmid+%23105011)/pmc13030966-223-20-28
Average 92 stars, based on 6 article reviews
pcag mito mtagbfp2 - by Bioz Stars,
2026-11
92/100 stars
Images
1) Product Images from "MitoTracker transfers from astrocytes to neurons independently of mitochondria"
Article Title: MitoTracker transfers from astrocytes to neurons independently of mitochondria
Journal: Cell Reports Methods
doi: 10.1016/j.crmeth.2026.101338
Figure Legend Snippet: Image acquisition immediately after astrocytes expressing GFP-OMM (green) and labeled with MitoTracker dye (magenta) are co-cultured with neurons expressing mito-mTagBFP2 (blue).
Techniques Used:
Figure Legend Snippet: Neurons expressing mTagBFP2 (blue) are incubated with ACM from astrocytes expressing GFP-OMM (green) and labeled with MitoTracker dye (magenta).
Techniques Used:
Figure Legend Snippet: MitoTracker, but not mitochondria, transfers rapidly to neurons from astrocytes (A) Mitochondria are dual labeled with outer mitochondrial membrane targeted GFP (GFP-OMM) and the MitoTracker dye. (B) Schematic diagram outlining the protocol for live co-culture experiments. Astrocytes are additionally labeled with CTDR (CellTracker Deep red) and neurons/neuronal mitochondria are identified by mTagBFP2 and mito-mTagBFP2 expression, respectively. (C) Timelapse images of a dual-labeled astrocyte (central) immediately following co-culture with neurons. ( n = 3 biological repeats, 4–5 positions of interest per repeat). (D) An orthogonal view of co-cultures following timelapse acquisition (>30 min). (E) Representative image of dual-labeled astrocytes co-cultured with mito-mTagBFP2 expressing neurons for >30 min. The yellow dashed line represents the astrocyte boundary. Insert highlights that MitoTracker but not GFP-OMM labels neuronal mitochondria. See also .
Techniques Used: Labeling, Membrane, Co-Culture Assay, Expressing, Cell Culture
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